Mussi A, Bonifati C, Carducci M, D'Agosto G, Pimpinelli F, D'Urso D, D'Auria L, Fazio M, Ameglio F
Laboratory of Clinical Pathology, S.Gallicano Institute, Roma, Italy.
J Biol Regul Homeost Agents. 1997 Jul-Sep;11(3):115-8.
TNF-alpha is a pleiotropic cytokine possibly involved in the pathogenesis of psoriasis.
to analyze the serum TNF-alpha levels in plaque-type psoriatic patients to evaluate the concentrations, correlation with the severity score and behaviour after therapy. The serum TNF-alpha levels of thirty-seven patients (25 females and 12 males; median age: 52.5 years, range 18-81: median PASI score: 11.4, range 3.5-42) and thirty healthy controls (21 females and 9 males, median age: 48.5 years, range 25-77) were compared after measurements obtained employing commercially available ELISA kits. The median serum TNF-alpha levels of the patients were significantly higher than those of controls (p = 0.004). 30/37 patients were followed over time at 2 and 4 weeks of treatment. Twenty one subjects were also observed after 6 weeks. After effective treatments, both the PASI scores and the cytokine levels were significantly and concomitantly reduced (p < 0.001). Significant correlations were found when the TNF-alpha values were plotted against the PASI scores both at the time of patient enrollment and at all the subsequent times (118 observations). A significant correlation was observed between circulating TNF-alpha and sE-selectin in agreement with a possible functional activity of the cytokine. However, no correlation was found between the cytokine levels and other 4 soluble membrane molecules. Our findings indicate that the molecule studied, although non specific for the disease considered, presents a behaviour paralleling that of the disease severity and therefore might have clinical usefulness, particularly in monitoring the therapeutic effects.
肿瘤坏死因子-α是一种多效性细胞因子，可能参与银屑病的发病机制。
分析斑块型银屑病患者血清肿瘤坏死因子-α水平，以评估其浓度、与严重程度评分的相关性以及治疗后的变化情况。使用市售酶联免疫吸附测定试剂盒进行检测后，比较了37例患者（25例女性和12例男性；中位年龄：52.5岁，范围18 - 81岁；中位银屑病面积和严重程度指数评分：11.4，范围3.5 - 42）和30名健康对照者（21例女性和9例男性，中位年龄：48.5岁，范围25 - 77岁）的血清肿瘤坏死因子-α水平。患者的血清肿瘤坏死因子-α中位水平显著高于对照组（p = 0.004）。30/37例患者在治疗2周和4周时进行了随访。21名受试者在6周后也进行了观察。有效治疗后，银屑病面积和严重程度指数评分及细胞因子水平均显著且同时降低（p < 0.001）。在患者入组时以及所有后续时间点（118次观察），将肿瘤坏死因子-α值与银屑病面积和严重程度指数评分绘制在一起时，发现了显著相关性。循环肿瘤坏死因子-α与可溶性E选择素之间观察到显著相关性，这与该细胞因子可能的功能活性一致。然而，细胞因子水平与其他4种可溶性膜分子之间未发现相关性。我们的研究结果表明，所研究的分子虽然对所考虑的疾病不具有特异性，但其表现与疾病严重程度平行，因此可能具有临床应用价值，特别是在监测治疗效果方面。
