Williams T
Southampton Public Health Laboratory, Southampton General Hospital, England, UK.
Br J Biomed Sci. 1997 Sep;54(3):181-5.
Pseudomonas aeruginosa is one of the most important bacterial pathogens in cystic fibrosis-associated lung disease. The means by which the organism is acquired or nosocomially spread is uncertain, but person-to-person spread has been implicated. Therefore, typing of P. aeruginosa from cystic fibrosis patients is necessary to determine the source of infection. Although P. aeruginosa strains can be compared using a variety of nucleic acid typing methods, this is time-consuming, expensive and requires specialised equipment, and is better suited to large reference laboratories. In this study, the use of antimicrobial sensitivity patterns in the investigation of possible cross-infection at cystic fibrosis clinics was examined, and 496 P. aeruginosa isolates from 69 patients were analysed. Analysis of dates and times of hospital visits allowed the study to concentrate on specific contact episodes where cross-infection could have occurred. Genotypic analysis of a number of organisms allowed the value of the sensitivity patterns to be assessed. No evidence of cross-infection was found, but antimicrobial sensitivity patterns proved unreliable in detecting similarity among P. aeruginosa strains.
铜绿假单胞菌是囊性纤维化相关肺部疾病中最重要的细菌病原体之一。该病原体的获得途径或医院内传播方式尚不确定，但已有证据表明存在人传人现象。因此，对囊性纤维化患者的铜绿假单胞菌进行分型对于确定感染源很有必要。虽然可以使用多种核酸分型方法对铜绿假单胞菌菌株进行比较，但这既耗时又昂贵，还需要专门的设备，更适合大型参考实验室。在本研究中，我们考察了利用抗菌药物敏感性模式来调查囊性纤维化诊所可能存在的交叉感染情况，并对69例患者的496株铜绿假单胞菌分离株进行了分析。通过分析医院就诊日期和时间，该研究能够集中关注可能发生交叉感染的特定接触事件。对多种微生物进行基因分型有助于评估敏感性模式的价值。虽然未发现交叉感染的证据，但抗菌药物敏感性模式在检测铜绿假单胞菌菌株之间的相似性方面被证明不可靠。
