孕妇对16型人乳头瘤病毒E4、E6和E7蛋白的血清学反应。

Serologic response to the E4, E6, and E7 proteins of human papillomavirus type 16 in pregnant women.

作者信息

Sethi S, Müller M, Schneider A, Blettner M, Smith E, Turek L, Wahrendorf J, Gissmann L, Chang-Claude J

机构信息

Deutsches Krebsforschungszentrum, Heidelberg, Germany.

出版信息

Am J Obstet Gynecol. 1998 Feb;178(2):360-4. doi: 10.1016/s0002-9378(98)80026-4.

DOI:10.1016/s0002-9378(98)80026-4

PMID:9500500

Abstract

OBJECTIVE

In a seroepidemiologic study the effects of pregnancy and other factors on humoral response to human papillomavirus type 16 infection were examined.

STUDY DESIGN

Multiple serum samples were taken at 3-month intervals for 15 months from 77 pregnant and 85 nonpregnant women. Serologic response to human papillomavirus type 16 proteins was analyzed with a peptide-based enzyme-linked immunosorbent assay.

RESULTS

Seroreactivity was higher in nonpregnant women than in pregnant women, suggesting a reduced humoral immune response against human papillomavirus infections during pregnancy. Among the pregnant women a twofold to threefold decrease in mean reactivity in the E4 protein-based assay was detected between early gestation and delivery. The presence of human papillomavirus type 16 or 18 deoxyribonucleic acid was significantly associated with reactivity to the E6 protein (p = 0.0005) and the E4 protein (p = 0.06). Reactivity to the E4 protein also correlated with an abnormal Papanicolaou smear.

CONCLUSIONS

The observation of changes in humoral response to genital human papillomavirus infections during pregnancy warrants further investigation with highly seroreactive assays.

摘要

目的

在一项血清流行病学研究中，检测妊娠及其他因素对人乳头瘤病毒16型感染的体液免疫反应的影响。

研究设计

从77名孕妇和85名非孕妇中，每隔3个月采集一次血清样本，共采集15个月。采用基于肽的酶联免疫吸附试验分析对人乳头瘤病毒16型蛋白的血清学反应。

结果

非孕妇的血清反应性高于孕妇，提示孕期对人乳头瘤病毒感染的体液免疫反应降低。在孕妇中，基于E4蛋白的检测中，妊娠早期至分娩期间平均反应性下降了两倍至三倍。人乳头瘤病毒16型或18型脱氧核糖核酸的存在与对E6蛋白（p = 0.0005）和E4蛋白（p = 0.06）的反应性显著相关。对E4蛋白的反应性也与巴氏涂片异常相关。