Pesold C, Impagnatiello F, Pisu M G, Uzunov D P, Costa E, Guidotti A, Caruncho H J
Psychiatric Institute, Department of Psychiatry, College of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA.
Proc Natl Acad Sci U S A. 1998 Mar 17;95(6):3221-6. doi: 10.1073/pnas.95.6.3221.
During embryonic development of brain laminated structures, the protein Reelin, secreted into the extracellular matrix of the cortex and hippocampus by Cajal-Retzius (CR) cells located in the marginal zone, contributes to the regulation of migration and positioning of cortical and hippocampal neurons that do not synthesize Reelin. Soon after birth, the CR cells decrease, and they virtually disappear during the following 3 weeks. Despite their disappearance, we can quantify Reelin mRNA (approximately 200 amol/ g of total RNA) and visualize it by in situ hybridization, and we detect the translated product of this mRNA by immunocytochemistry preferentially in gamma-aminobutyric acid (GABA)ergic neurons of adult rat cortex and hippocampus. In adult rat cerebellum, Reelin is expressed in glutamatergic neurons (granule cells). The translated product of this mRNA is readily exported from the granule cell somata to the parallel fibers, where it has been detected by electron microscopy in axon terminals located presynaptically to Purkinje cell dendrites.
在脑分层结构的胚胎发育过程中，位于边缘区的Cajal-Retzius（CR）细胞分泌到皮质和海马细胞外基质中的Reelin蛋白，有助于调节不合成Reelin的皮质和海马神经元的迁移和定位。出生后不久，CR细胞数量减少，在接下来的3周内几乎消失。尽管它们消失了，但我们可以定量Reelin mRNA（约200 amol/g总RNA）并通过原位杂交对其进行可视化，并且我们通过免疫细胞化学优先在成年大鼠皮质和海马的γ-氨基丁酸（GABA）能神经元中检测到该mRNA的翻译产物。在成年大鼠小脑中，Reelin在谷氨酸能神经元（颗粒细胞）中表达。该mRNA的翻译产物很容易从颗粒细胞胞体输出到平行纤维，在那里通过电子显微镜在浦肯野细胞树突突触前的轴突终末中检测到它。
