Alcántara S, Ruiz M, D'Arcangelo G, Ezan F, de Lecea L, Curran T, Sotelo C, Soriano E
Department of Animal and Plant Cell Biology, Faculty of Biology, University of Barcelona, Barcelona 08028, Spain.
J Neurosci. 1998 Oct 1;18(19):7779-99. doi: 10.1523/JNEUROSCI.18-19-07779.1998.
The reelin gene encodes an extracellular protein that is crucial for neuronal migration in laminated brain regions. To gain insights into the functions of Reelin, we performed high-resolution in situ hybridization analyses to determine the pattern of reelin expression in the developing forebrain of the mouse. We also performed double-labeling studies with several markers, including calcium-binding proteins, GAD65/67, and neuropeptides, to characterize the neuronal subsets that express reelin transcripts. reelin expression was detected at embryonic day 10 and later in the forebrain, with a distribution that is consistent with the prosomeric model of forebrain regionalization. In the diencephalon, expression was restricted to transverse and longitudinal domains that delineated boundaries between neuromeres. During embryogenesis, reelin was detected in the cerebral cortex in Cajal-Retzius cells but not in the GABAergic neurons of layer I. At prenatal stages, reelin was also expressed in the olfactory bulb, and striatum and in restricted nuclei in the ventral telencephalon, hypothalamus, thalamus, and pretectum. At postnatal stages, reelin transcripts gradually disappeared from Cajal-Retzius cells, at the same time as they appeared in subsets of GABAergic neurons distributed throughout neocortical and hippocampal layers. In other telencephalic and diencephalic regions, reelin expression decreased steadily during the postnatal period. In the adult, there was prominent expression in the olfactory bulb and cerebral cortex, where it was restricted to subsets of GABAergic interneurons that co-expressed calbindin, calretinin, neuropeptide Y, and somatostatin. This complex pattern of cellular and regional expression is consistent with Reelin having multiple roles in brain development and adult brain function.
reelin基因编码一种细胞外蛋白，该蛋白对分层脑区的神经元迁移至关重要。为深入了解Reelin的功能，我们进行了高分辨率原位杂交分析，以确定reelin在小鼠发育中的前脑的表达模式。我们还使用了几种标记物进行双标记研究，包括钙结合蛋白、GAD65/67和神经肽，以表征表达reelin转录本的神经元亚群。在胚胎第10天及以后在前脑中检测到reelin表达，其分布与前脑区域化的前体模型一致。在间脑中，表达局限于划分神经节之间边界的横向和纵向区域。在胚胎发生过程中，在大脑皮层的Cajal-Retzius细胞中检测到reelin，但在I层的GABA能神经元中未检测到。在产前阶段，reelin也在嗅球、纹状体以及腹侧端脑、下丘脑、丘脑和顶盖前区的特定核团中表达。在出生后阶段，reelin转录本逐渐从Cajal-Retzius细胞中消失，与此同时，它们出现在分布于整个新皮层和海马层的GABA能神经元亚群中。在其他端脑和间脑区域，reelin表达在出生后阶段稳步下降。在成体中，在嗅球和大脑皮层中有显著表达，其中它局限于共表达钙结合蛋白、钙视网膜蛋白、神经肽Y和生长抑素的GABA能中间神经元亚群。这种复杂的细胞和区域表达模式与Reelin在脑发育和成年脑功能中具有多种作用一致。
