Baldock P A, Morris H A, Moore R J, Need A G, Durbridge T C
Department of Physiology, University of Adelaide, South Australia.
Calcif Tissue Int. 1998 Mar;62(3):244-9. doi: 10.1007/s002239900424.
Bone loss after oophorectomy of adult rats is more rapid and complete in the metaphysis than in the epiphysis of the femur, particularly in the proximal region of the metaphysis distant from the growth plate. This study was undertaken to determine the effects of prepubertal oophorectomy, on femoral cancellous bone acquisition during growth. Rats were oophorectomized (OVX) or sham operated at 3 weeks of age and killed at intervals up to 78 weeks for scanning electron microscopy and histomorphometry of the distal femur. Differences in cancellous bone architecture between the two groups was evident after 6 weeks of age. Relatively minor differences were found in the part of the metaphysis near the growth plate and in the epiphysis, with less trabeculae in the primary spongiosa and 1 to 2 less trabeculae/mm in the secondary spongiosa. However, as metaphyseal growth proceeded, trabeculae were present for a greater distance up the femoral shaft in controls than in OVX rats, with mean BV/TV in the proximal part of the metaphysis increasing from 1.4% at 6 weeks to 13.4% at 20 weeks in controls, with no increase in the OVX rats. We find that the lack of ovarian hormones increases the rate of destruction of trabeculae near the metaphyseal-diaphyseal junction.
成年大鼠卵巢切除术后，股骨干骺端的骨质流失比骨骺更快且更完全，尤其是在远离生长板的干骺端近端区域。本研究旨在确定青春期前卵巢切除对生长过程中股骨松质骨获取的影响。大鼠在3周龄时接受卵巢切除术（OVX）或假手术，并在78周内定期处死，以对股骨远端进行扫描电子显微镜检查和组织形态计量学分析。两组之间的松质骨结构差异在6周龄后明显。在靠近生长板的干骺端部分和骨骺中发现相对较小的差异，初级松质骨中的小梁较少，次级松质骨中的小梁每毫米少1至2根。然而，随着干骺端生长的进行，与OVX大鼠相比，对照组股骨近端干骺端的小梁在股骨干上的延伸距离更长，对照组干骺端近端的平均骨体积/组织体积从6周时的1.4%增加到20周时的13.4%，而OVX大鼠则没有增加。我们发现，缺乏卵巢激素会增加干骺端-骨干交界处附近小梁的破坏速度。
