Durbridge T C, Morris H A, Parsons A M, Parkinson I H, Moore R J, Porter S, Need A G, Nordin B E, Vernon-Roberts B
Institute of Medical and Veterinary Science, IMVS Division of Tissue Pathology, Adelaide, South Australia.
Calcif Tissue Int. 1990 Dec;47(6):383-7. doi: 10.1007/BF02555891.
Female Sprague-Dawley rats (n = 72), 6 months old, underwent either sham operation, oophorectomy, adrenalectomy, or combined oophorectomy and adrenalectomy (O&A). They were all maintained on normal saline ad libitum and 20 g/day 1.1% calcium chow. Nine weeks after operation, the trabecular bone volume of the distal femoral shaft was significantly lower (P less than 0.001) in the adrenalectomized (11.1%), oophorectomized (7.0%), and O&A (8.3%) animals than in sham-operated animals (19.8%). Eighteen weeks after operation, the trabecular bone volume in O&A animals had fallen to a mean of 3.8% (sham 17.0%), and the length of the femur had increased to 38.8 mm after O&A (sham 36.8 mm, P less than 0.01). O&A animals treated with 0.35 mg/kg/week nandrolone decanoate from 9 weeks postoperatively onward, had twice the femoral trabecular bone volume of untreated animals at 18 weeks (P less than 0.05). By contrast, no significant differences were found in vertebral body trabecular bone between any groups, including groups receiving treatment with androgens. We have found that, by 9 weeks after operation, adrenalectomy alone causes significant loss of metaphyseal trabecular bone, similar to the progressive loss seen after oophorectomy.
6月龄雌性Sprague-Dawley大鼠（n = 72）接受假手术、卵巢切除术、肾上腺切除术或卵巢切除与肾上腺切除联合手术（卵巢与肾上腺联合切除）。它们均自由饮用生理盐水，并每日给予20克1.1%的钙饲料。术后9周，肾上腺切除组（11.1%）、卵巢切除组（7.0%）和卵巢与肾上腺联合切除组（8.3%）动物股骨干远端的小梁骨体积显著低于假手术组动物（19.8%）（P < 0.001）。术后18周，卵巢与肾上腺联合切除组动物的小梁骨体积降至平均3.8%（假手术组为17.0%），且卵巢与肾上腺联合切除组动物股骨长度增加至38.8毫米（假手术组为36.8毫米，P < 0.01）。从术后9周起接受每周0.35毫克/千克癸酸诺龙治疗的卵巢与肾上腺联合切除组动物，在18周时股骨小梁骨体积是未治疗动物的两倍（P < 0.05）。相比之下，包括接受雄激素治疗的组在内，各实验组椎体小梁骨未见显著差异。我们发现，术后9周时，单纯肾上腺切除就会导致干骺端小梁骨显著丢失，与卵巢切除后逐渐出现的骨量丢失相似。
