McGaw M M, Chandler L J, Wasieloski L P, Blair C D, Beaty B J
Department of Microbiology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80523, USA.
Am J Trop Med Hyg. 1998 Feb;58(2):168-75. doi: 10.4269/ajtmh.1998.58.168.
The effect of La Crosse (LAC) virus infection on Aedes triseriatus overwintering success was determined. Eggs from LAC virus transovarially infected (LAC TOT+) and uninfected (LAC TOT-) Ae. triseriatus colonies were induced into diapause, held in natural conditions, and returned to the laboratory at predetermined times for assay of diapause, mortality, and filial infection rates, and to examine viral transcription and replication during diapause. Embryos from the LAC TOT+ colony exhibited greater cumulative mortality (16.7%) than the LAC TOT- eggs (7.3%) throughout the overwintering periods. The increased mortality rate in LAC TOT+ eggs corresponded with a decrease in filial infection rates. Eggs from the LAC TOT+ colony terminated diapause more readily than the LAC TOT- colony. An RNA strand-specific reverse transcriptase-polymerase chain reaction technique was used to monitor viral transcription and replication in mosquito eggs during overwintering, and to compare viral replication in diapausing and nondiapausing embryos. Viral messenger and replicative form RNA were present in eggs in all sample periods, suggesting that some virus replication occurred during diapause.
研究了拉克罗斯（LAC）病毒感染对三带喙库蚊越冬成功率的影响。从经卵传递感染LAC病毒（LAC TOT+）和未感染（LAC TOT-）的三带喙库蚊群体中获取的卵被诱导进入滞育状态，置于自然条件下，并在预定时间返回实验室，以检测滞育、死亡率和子代感染率，并研究滞育期间病毒的转录和复制情况。在整个越冬期间，来自LAC TOT+群体的胚胎表现出比LAC TOT-卵更高的累积死亡率（16.7%）（7.3%）。LAC TOT+卵死亡率增加与子代感染率下降相对应。来自LAC TOT+群体的卵比LAC TOT-群体的卵更容易终止滞育。采用RNA链特异性逆转录-聚合酶链反应技术监测越冬期间蚊卵中的病毒转录和复制，并比较滞育和非滞育胚胎中的病毒复制情况。在所有采样期的卵中均存在病毒信使RNA和复制型RNA，这表明在滞育期间发生了一些病毒复制。
