Crain P F, McCloskey J A
University of Utah, Department of Medicinal Chemistry, Salt Lake City 84112-5820, USA.
Curr Opin Biotechnol. 1998 Feb;9(1):25-34. doi: 10.1016/s0958-1669(98)80080-3.
Mass spectrometry-based techniques continue to undergo active development for applications to nucleic acids, fueled by methods based on electrospray and matrix-assisted laser desorption ionization. In the past two years, notable advances have occurred in multiple interrelated areas, including sequencing techniques for oligonucleotides, approaches to mixture analysis, microscale sample handling and targeted DNA assays, and improvements in instrumentation for greater sensitivity and mass resolution.
基于质谱的技术在用于核酸分析方面持续得到积极发展，这得益于基于电喷雾和基质辅助激光解吸电离的方法。在过去两年中，多个相互关联的领域取得了显著进展，包括寡核苷酸测序技术、混合物分析方法、微尺度样品处理和靶向DNA检测，以及提高灵敏度和质量分辨率的仪器改进。
