质谱分析法在基因组学中的应用。

The use of mass spectrometry in genomics.

作者信息

Meng Zhaojing, Simmons-Willis Tracey A, Limbach Patrick A

机构信息

Rieveschl Laboratories for Mass Spectrometry, Department of Chemistry, University of Cincinnati, PO Box 210172, Cincinnati, OH 45221-0172, USA.

出版信息

Biomol Eng. 2004 Jan;21(1):1-13. doi: 10.1016/j.bioeng.2003.08.001.

DOI:10.1016/j.bioeng.2003.08.001

PMID:14715314

Abstract

Recent advances in the development of electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI) now permit the near routine analysis of oligonucleotides and intact nucleic acids. These developments have led to the use of mass spectrometry (MS) as a detection platform for genomics studies. Among the various uses of mass spectrometry in genomics, applications focused on the characterization of single nucleotide polymorphisms (SNPs) and short tandem repeats (STRs) are particularly well-suited to MALDI or ESI-based analysis. It is predicted that continued developments in methodology and instrumentation will further improve the capabilities of mass spectrometry for nucleic acid analysis.

摘要

电喷雾电离（ESI）和基质辅助激光解吸/电离（MALDI）技术的最新进展，如今已使寡核苷酸和完整核酸的近乎常规分析成为可能。这些进展促使质谱（MS）成为基因组学研究的检测平台。在质谱在基因组学的各种应用中，专注于单核苷酸多态性（SNP）和短串联重复序列（STR）表征的应用尤其适合基于MALDI或ESI的分析。据预测，方法学和仪器设备的持续发展将进一步提升质谱用于核酸分析的能力。