Griffiths A D, Duncan A R
MRC Laboratory of Molecular Biology, Cambridge, UK.
Curr Opin Biotechnol. 1998 Feb;9(1):102-8. doi: 10.1016/s0958-1669(98)80092-x.
Phage antibody-display is rapidly maturing into a very effective tool for antibody generation. The recent development of large primary antibody libraries enables selection of antibodies against most targets in under two weeks and many of these antibodies have relatively high (nanomolar) affinities. Successful strategies have also been developed to affinity mature these antibodies into the picomolar range if required.
噬菌体抗体展示正迅速发展成为一种非常有效的抗体生成工具。大型初级抗体文库的最新进展使得能够在两周内筛选出针对大多数靶点的抗体，而且其中许多抗体具有相对较高（纳摩尔级）的亲和力。如果需要，还开发出了成功的策略将这些抗体的亲和力成熟至皮摩尔级范围。
