吡咯表征中的自动转移色谱法。多斑点现象的化学性质。

Autotransfer chromatography in the characterization of pyrroles. Chemistry of multiple-spot phenomena.

作者信息

Irvine D G

出版信息

J Chromatogr. 1976 Jul 21;123(1):69-78. doi: 10.1016/s0021-9673(00)81104-8.

Abstract

A characteristic pattern of five interconverting "component" zones is obtained on autotransfer chromatography when pure kryptopyrrole (KP) is adsorbed from aqueous solution onto charcoal and then eluted with methanol. Specific oxidation products (lactams) derived from synthetic KP matched four of the five zones. Zone 1 was obtained directly from the a'-hydroxy-a-lactam. Zone 3 was produced directly by the a'-methoxy-a-lactam, while zones 2 and 5 were formed as a consequence of its acid hydrolysis during equilibration and chromatographic development. The formation of the methoxy lactam itself depended upon the presence of added methanol in the extraction process. In the absence of methanol during extraction only zone 1 (sometimes accompanied by zone 4) was formed.

摘要

当将纯隐卟啉（KP）从水溶液吸附到活性炭上，然后用甲醇洗脱时，在自动转移色谱法中可获得由五个相互转化的“组分”区组成的特征模式。合成KP衍生的特定氧化产物（内酰胺）与五个区中的四个相匹配。区1直接由α'-羟基-α-内酰胺得到。区3直接由α'-甲氧基-α-内酰胺产生，而区2和区5是在平衡和色谱展开过程中其酸水解的结果。甲氧基内酰胺本身的形成取决于萃取过程中添加甲醇的存在。在萃取过程中不存在甲醇时，仅形成区1（有时伴有区4）。