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[免疫复合物中分枝杆菌抗原的测定]

[The determination of mycobacterial antigens found in immune complexes].

作者信息

Baenskiĭ A V, Dem'ianenko N V, Kalinina O A, Sergeeva L V, Litvinov V I

出版信息

Probl Tuberk. 1993(6):5-7.

PMID:8290535
Abstract

By using affine isolation from hyperimmune rabbit sera on homologous mycobacteria humanus (H37Rv) and affine purification on atypical mycobacteria and BCG mycobacteria, the authors obtained a preparation of antituberculous antibodies. These were used in indirect ELISA to detect antigens (after IC dissociation) in tuberculous patients. Out of 70 patients with infiltrative pulmonary tuberculosis, 30 patients with other pulmonary diseases and 30 healthy donors the antigens were found in 77.1% of tuberculous cases and in 6.7% of healthy subjects. The results of the test say in favour of its benefits in practical application. The antigens levels and incidence of positive reaction are higher in patients with destructive tuberculosis and in the bacterial discharge.

摘要

通过用来自超免疫兔血清对同源人分枝杆菌(H37Rv)进行亲和分离,并对非典型分枝杆菌和卡介苗分枝杆菌进行亲和纯化,作者获得了抗结核抗体制剂。这些抗体用于间接酶联免疫吸附测定法(ELISA)以检测结核病患者中的抗原(免疫复合物解离后)。在70例浸润性肺结核患者、30例其他肺部疾病患者和30名健康供者中,77.1%的结核病例和6.7%的健康受试者检测到了抗原。检测结果表明其在实际应用中具有优势。在有破坏性肺结核和细菌排出的患者中,抗原水平和阳性反应发生率更高。

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