犬心脏蛋白质的HSC - 2D PAGE及二维凝胶电泳数据库

HSC-2DPAGE and the two-dimensional gel electrophoresis database of dog heart proteins.

作者信息

Dunn M J, Corbett J M, Wheeler C H

机构信息

Cardiothoracic Surgery, National Heart and Lung Institute, Imperial College School of Medicine, Harefield Hospital, UK.

出版信息

Electrophoresis. 1997 Dec;18(15):2795-802. doi: 10.1002/elps.1150181514.

DOI:10.1002/elps.1150181514

PMID:9504812

Abstract

A two-dimensional gel electrophoresis database of dog (Canis familiaris) proteins is presented. The database contains 1212 protein spots which have been characterised in terms of their pI and Mr. This database has been integrated into the HSC-2DPAGE database which is accessible on the Internet via the World Wide Web with the uniform resource location (URL): (http://www.harefield.nthames.nhs.uk/nhli/ protein/index.html). Identifications for 80 of the protein spots have been obtained by visual cross-matching with the human heart protein database in HSC-2DPAGE (42 spots), N-terminal microsequence analysis (25 spots) and peptide mass fingerprinting (20 spots). This database is being used in studies of alterations in protein expression in models of heart failure and heart disease.

摘要

本文展示了犬（犬属）蛋白质的二维凝胶电泳数据库。该数据库包含1212个已根据其等电点（pI）和相对分子质量（Mr）进行表征的蛋白质斑点。此数据库已整合到HSC - 2DPAGE数据库中，可通过万维网在互联网上通过统一资源定位符（URL）访问：(http://www.harefield.nthames.nhs.uk/nhli/protein/index.html)。通过与HSC - 2DPAGE中的人类心脏蛋白质数据库进行视觉交叉匹配（42个斑点）、N端微序列分析（25个斑点）和肽质量指纹图谱分析（20个斑点），已获得80个蛋白质斑点的鉴定结果。该数据库正用于心力衰竭和心脏病模型中蛋白质表达变化的研究。