Evans G, Wheeler C H, Corbett J M, Dunn M J
National Heart and Lung Institute, Imperial College School of Medicine, Harefield Hospital, UK.
Electrophoresis. 1997 Mar-Apr;18(3-4):471-9. doi: 10.1002/elps.1150180322.
The dissemination of information relating to the characterisation of proteins from two-dimensional electrophoresis (2-DE) gel databases is essential for their effective utilisation in the study of protein expression in cell biology. Since the inception of the World Wide Web and the pioneering development of SWISS-2DPAGE as a tool for retrieving information on proteins separated by 2-DE, the Internet has become the method of choice for disseminating and accessing information on 2-DE protein databases. At Harefield we have established HSC-2DPAGE which is an advanced interface for accessing protein database relating to heart disease. The Web site currently includes databases of proteins from human, dog and rat ventricular tissue and a human endothelial cell line. The databases are searchable individually or as a whole by remote keyword searches. Each database is represented by both synthetic (computer generated) and real (scanned gel) clickable images upon which characterised protein spots are highlighted by hyperlinked symbols. The database conforms to all the rules proposed for federated 2-DE protein databases and individual protein entries are linked to other protein databases such as SWISS-PROT by active cross-references. This paper describes the construction of HSC-2DPAGE, its maintenance, and access via the Internet.
从二维电泳（2-DE）凝胶数据库中传播有关蛋白质特征的信息，对于其在细胞生物学蛋白质表达研究中的有效利用至关重要。自从万维网诞生以及SWISS-2DPAGE作为检索二维电泳分离蛋白质信息的工具取得开创性发展以来，互联网已成为传播和获取二维电泳蛋白质数据库信息的首选方法。在哈雷菲尔德，我们建立了HSC-2DPAGE，它是一个用于访问与心脏病相关蛋白质数据库的高级界面。该网站目前包括来自人类、狗和大鼠心室组织以及人类内皮细胞系的蛋白质数据库。这些数据库可通过远程关键词搜索单独或整体进行搜索。每个数据库都由合成（计算机生成）和真实（扫描凝胶）的可点击图像表示，其上通过超链接符号突出显示已表征的蛋白质斑点。该数据库符合为联合二维电泳蛋白质数据库提议的所有规则，并且单个蛋白质条目通过活跃的交叉引用链接到其他蛋白质数据库，如SWISS-PROT。本文描述了HSC-2DPAGE的构建、维护以及通过互联网的访问。
