Zambonin G, Colucci S, Cantatore F, Grano M
Ist Orthopaedic Clinic, University of Bari, Bari, Italy.
Calcif Tissue Int. 1998 Apr;62(4):362-5. doi: 10.1007/s002239900445.
The effects of a polymethylmetacrylate (PMMA) powder with a diameter between 0.5 and 25 mu m have been studied in vitro on several human osteoblast populations obtained from different sources. Parameters of cell activity such as cell growth, collagen synthesis, osteocalcin, and interleukin-6 (IL-6) production have been evaluated. Cell proliferation and collagen synthesis were inhibited after exposure to bone cement, whereas osteocalcin and IL-6 production were stimulated. These results suggest that PMMA particles could affect osteoblast activity in a way that could contribute, together with other factors, to periprosthetic osteolysis through two different pathways: a reduced periprosthetic bone formation due to the reduced osteoblast proliferation and collagen synthesis, and an osteoblast-mediated activation of osteoclastic bone resorption as suggested by the increased osteocalcin and IL-6 synthesis. In fact, osteocalcin has been demonstrated to have a role in osteoclast recruitment to bone surfaces, and IL-6 is known to induce osteoclastogenesis and to directly stimulate bone resorption.
已在体外研究了直径在0.5至25微米之间的聚甲基丙烯酸甲酯（PMMA）粉末对从不同来源获取的几个人类成骨细胞群体的影响。已评估了细胞活性参数，如细胞生长、胶原蛋白合成、骨钙素和白细胞介素-6（IL-6）的产生。暴露于骨水泥后，细胞增殖和胶原蛋白合成受到抑制，而骨钙素和IL-6的产生则受到刺激。这些结果表明，PMMA颗粒可能以一种与其他因素共同作用，通过两种不同途径导致假体周围骨溶解的方式影响成骨细胞活性：由于成骨细胞增殖和胶原蛋白合成减少，假体周围骨形成减少；骨钙素和IL-6合成增加表明成骨细胞介导破骨细胞性骨吸收激活。事实上，已证明骨钙素在破骨细胞募集到骨表面中起作用，并且已知IL-6可诱导破骨细胞生成并直接刺激骨吸收。
