Russo L G, Maharajan P, Maharajan V
Department of Structure, Function and Biotechnology, Faculty of Veterinary Medicine, University of Naples Federico II, Italy.
Growth Factors. 1998;15(2):125-33. doi: 10.3109/08977199809117188.
This study describes the spatio-temporal expression of basic Fibroblast growth factor (FGF-2) during odontogenesis of mouse as revealed by immunohistology. Parasagittal sections of mouse embryo head (13-18 day of gestation) containing various stages of developing tooth were incubated with a polyclonal anti-FGF-2 antibody and positive binding was evidentiated by using Streptavidin-Biotin complex-HRP system and AEC staining. We observed no FGF-2 staining at the dental lamina stage. At the bud stage slight staining is seen, limited to some epithelial cells. The intensity of the staining increases at the cap stage. In the bell stage, the stellate reticulum cells stain intensely. Later, odontoblasts and the dentin matrix stain deeply; but the epithelial cells stain faint. The extra cellular matrix of the dentin and dental papilla stain very intense but the enamel matrix is found negative. These results indicate the participation of FGF-2 in differentiation rather than in proliferation of tooth-forming cells. In particular, it appears that FGF-2 participates in odontoblast differentiation and in dentin matrix deposition. The spatio-temporally specific distribution pattern of FGF-2 in developing mouse tooth reported here emphasizes the importance of FGF-2 in mammalian odontogenesis.
本研究通过免疫组织学揭示了小鼠牙胚发生过程中碱性成纤维细胞生长因子（FGF - 2）的时空表达。将包含不同发育阶段牙齿的小鼠胚胎头部矢状旁切片（妊娠13 - 18天）与多克隆抗FGF - 2抗体孵育，并使用链霉亲和素 - 生物素复合物 - HRP系统和AEC染色来显示阳性结合。在牙板阶段未观察到FGF - 2染色。在蕾状期可见轻微染色，局限于一些上皮细胞。帽状期染色强度增加。在钟状期，星网状细胞染色强烈。之后，成牙本质细胞和牙本质基质染色深；但上皮细胞染色浅。牙本质和牙髓乳头的细胞外基质染色非常强烈，但釉质基质呈阴性。这些结果表明FGF - 2参与牙形成细胞的分化而非增殖。特别是，FGF - 2似乎参与成牙本质细胞分化和牙本质基质沉积。本文报道的FGF - 2在发育中小鼠牙齿中的时空特异性分布模式强调了FGF - 2在哺乳动物牙胚发生中的重要性。
