Sagomonyants Karen, Mina Mina
Department of Craniofacial Sciences, School of Dental Medicine, University of Connecticut Health Center , Farmington, CT , USA.
Connect Tissue Res. 2014 Aug;55 Suppl 1(0 1):53-6. doi: 10.3109/03008207.2014.923867.
Odontoblast differentiation during physiological and reparative dentinogenesis is dependent upon multiple signaling molecules, including fibroblast growth factors (FGFs), bone morphogenetic proteins (BMPs) and Wingless/Integrated (Wnt) ligands. Recent studies in our laboratory showed that continuous exposure of primary dental pulp cultures to FGF2 exerted biphasic effects on the expression of markers of dentinogenesis. In the present study, we examined the possible involvement of the BMP and Wnt signaling pathways in mediating the effects of FGF2 on dental pulp cells. Our results showed that stimulatory effects of FGF2 on dentinogenesis during the proliferation phase of growth were associated with increased expression of the components of the BMP (Bmp2, Dlx5, Msx2, Osx) and Wnt (Wnt10a, Wisp2) pathways, and decreased expression of an inhibitor of the Wnt signaling, Nkd2. Further addition of FGF2 during the differentiation/mineralization phase of growth resulted in decreased expression of components of the BMP signaling (Bmp2, Runx2, Osx) and increased expression of inhibitors of the Wnt signaling (Nkd2, Dkk3). This suggests that both BMP and Wnt pathways may be involved in mediating the effects of FGF2 on dental pulp cells.
在生理性和修复性牙本质形成过程中，成牙本质细胞的分化依赖于多种信号分子，包括成纤维细胞生长因子（FGFs）、骨形态发生蛋白（BMPs）和无翅型/整合型（Wnt）配体。我们实验室最近的研究表明，将原代牙髓培养物持续暴露于FGF2对牙本质形成标志物的表达具有双相作用。在本研究中，我们研究了BMP和Wnt信号通路在介导FGF2对牙髓细胞作用中的可能参与情况。我们的结果表明，在生长的增殖阶段，FGF2对牙本质形成的刺激作用与BMP（Bmp2、Dlx5、Msx2、Osx）和Wnt（Wnt10a、Wisp2）通路成分的表达增加以及Wnt信号抑制剂Nkd2的表达降低有关。在生长的分化/矿化阶段进一步添加FGF2导致BMP信号通路成分（Bmp2、Runx2、Osx）的表达降低以及Wnt信号抑制剂（Nkd2、Dkk3）的表达增加。这表明BMP和Wnt通路可能都参与介导FGF2对牙髓细胞的作用。
