Gubskiĭ Iu I, Goriushko A G, Shnurko-Tabakova E V
Institute of Pharmacology and Toxicology, Academy of Medical Sciences of Ukraine, Kyiv.
Ukr Biokhim Zh (1978). 1997 May-Jun;69(3):60-5.
Interaction of phenol type antioxidants and Ca-channel blockers, derivatives of 1,4-dihydropyridines, with rat liver microsomes has been studied in vitro by means of spectrophotometry and fluorescent probes using ANS and polymethine dye 4501 u. Incubation of microsomes in the absorption spectra of the antioxidant results in changes of structure dynamic parametres of microsomal membranes, and in changes of their microviscosity and in surface state modification. The mechanism of microsomal action was different for phenol type antioxidants and for 1,4-dihydropyridines and depended on the radical structure. Ability of Ca-channel blockers and verapamil to interact with microsomal membrane protein structures was shown.
通过使用ANS和聚甲炔染料4501 u的分光光度法和荧光探针，在体外研究了酚类抗氧化剂与1,4 - 二氢吡啶衍生物钙通道阻滞剂与大鼠肝微粒体的相互作用。在抗氧化剂的吸收光谱中对微粒体进行孵育，会导致微粒体膜结构动力学参数的变化、微粘度的变化以及表面状态的改变。酚类抗氧化剂和1,4 - 二氢吡啶对微粒体的作用机制不同，且取决于自由基结构。研究表明钙通道阻滞剂和维拉帕米能够与微粒体膜蛋白结构相互作用。
