Armitage L L, Mohapel P, Jenkins E M, Hannesson D K, Corcoran M E
University Of Victoria, Department of Psychology, PO Box 3050, Victoria, BC, Canada.
Brain Res. 1998 Jan 19;781(1-2):37-44. doi: 10.1016/s0006-8993(97)01218-3.
In an attempt to determine whether sprouting of mossy fibers is invariably correlated with kindling of seizures, we subjected rats to rapid kindling with long trains of low-frequency stimulation of the amygdala that resulted in development of generalized seizures within a mean of five stimulations. For comparison, we subjected other rats to conventional kindling with short trains of high-frequency stimulation of the amygdala that resulted in development of generalized seizures within a mean of 13 stimulations. We found no evidence of mossy fiber sprouting in the dentate gyrus of rats killed one day after completion of rapid kindling, as compared to yoked controls, although significant sprouting was seen in rats killed one day after completion of conventional kindling. When we examined tissue from rats killed 20 days after rapid kindling, however, we did find significant sprouting, suggesting that mossy fiber sprouting can be triggered by rapid kindling if sufficient survival time is allowed. The observed disparity between completion of rapid low-frequency kindling and detection of mossy fiber sprouting suggests that mossy fiber sprouting may be associated more with sustained survival time after neuronal activation than with kindling per se. Furthermore, the similar time course of conventional kindling and of mossy fiber sprouting obscures the determination of a causal role of mossy fiber sprouting in conventional kindling.
为了确定苔藓纤维出芽是否总是与癫痫发作的点燃相关，我们对大鼠进行了快速点燃，采用长串的杏仁核低频刺激，这导致在平均五次刺激内出现全身性癫痫发作。作为对照，我们对其他大鼠进行了传统点燃，采用短串的杏仁核高频刺激，这导致在平均13次刺激内出现全身性癫痫发作。与配对对照相比，我们发现在快速点燃完成后一天处死的大鼠齿状回中没有苔藓纤维出芽的证据，尽管在传统点燃完成后一天处死的大鼠中观察到了明显的出芽。然而，当我们检查快速点燃后20天处死的大鼠组织时，我们确实发现了明显的出芽，这表明如果给予足够的存活时间，快速点燃可以触发苔藓纤维出芽。快速低频点燃完成与苔藓纤维出芽检测之间观察到的差异表明，苔藓纤维出芽可能更多地与神经元激活后的持续存活时间有关，而不是与点燃本身有关。此外，传统点燃和苔藓纤维出芽的相似时间进程掩盖了确定苔藓纤维出芽在传统点燃中的因果作用。
