Armstrong N J, Steinbeisser H, Prothmann C, DeLotto R, Rupp R A
Friedrich Miescher Laboratorium, MPG, Spemannstrasse 37-39, 72076, Tubingen, Germany.
Mech Dev. 1998 Feb;71(1-2):99-105. doi: 10.1016/s0925-4773(98)00003-3.
The Spätzle/Toll signaling pathway controls ventral axis formation in Drosophila by generating a gradient of nuclear Dorsal protein. Dorsal controls the downstream regulators dpp and sog, whose patterning functions are conserved between insects and vertebrates. Although there is no experimental evidence that the upstream events are conserved as well, we set out to ask if a vertebrate embryo can respond to maternal components of the fly Dorsal pathway. Here we demonstrate a dorsalizing activity for the heterologous Easter, Spätzle and Toll proteins in UV-ventralized Xenopus embryos, which is inhibited by a co-injected dominant Cactus variant. We conclude that the Dorsal signaling pathway is a component of the conserved dorsoventral (d/v) patterning system in bilateria.
斯佩茨尔/托勒信号通路通过产生核背蛋白梯度来控制果蝇腹侧轴的形成。背蛋白控制下游调节因子dpp和sog，它们在昆虫和脊椎动物之间的模式形成功能是保守的。尽管没有实验证据表明上游事件也具有保守性，但我们着手研究脊椎动物胚胎是否能对果蝇背信号通路的母体成分做出反应。在这里，我们证明了异源伊斯特、斯佩茨尔和托勒蛋白在紫外线腹侧化的非洲爪蟾胚胎中具有背侧化活性，这种活性被共注射的显性仙人掌变体所抑制。我们得出结论，背信号通路是两侧对称动物中保守的背腹（d/v）模式形成系统的一个组成部分。
