Zine A, de Ribaupierre F
Institut de Physiologie, Lausanne, Switzerland.
Neuroreport. 1998 Jan 26;9(2):263-8. doi: 10.1097/00001756-199801260-00016.
We investigated the potential for hair cell regeneration in neonatal rat organs of Corti grown in culture following destruction of hair cells by neomycin toxicity. Replacement hair cells were observed by light and scanning electron microscopy in lesion sites in the cultures treated with transforming growth factor-alpha, epidermal growth factor or a combination of both factors for 5-7 days post-injury. These new cells had morphological characteristics of immature hair cells. Autoradiographic localization of [3H]thymidine-labelled cells on semi-thin sections indicated that these replacement hair cells did not arise through renewed mitotic division, although an important mitotic proliferation was observed outside the area of supporting and sensory cells.
我们研究了新生大鼠柯蒂氏器在新霉素毒性破坏毛细胞后于培养中生长时毛细胞再生的可能性。在用转化生长因子-α、表皮生长因子或这两种因子的组合处理损伤后5 - 7天的培养物的损伤部位，通过光学显微镜和扫描电子显微镜观察到了替代毛细胞。这些新细胞具有未成熟毛细胞的形态特征。[3H]胸腺嘧啶核苷标记细胞在半薄切片上的放射自显影定位表明，这些替代毛细胞并非通过重新进行有丝分裂产生，尽管在支持细胞和感觉细胞区域之外观察到了重要的有丝分裂增殖。
