通过体外玻璃黏附试验鉴别不同毒力水平的肠炎沙门氏菌菌株。
Discrimination of strains of Salmonella enteritidis with differing levels of virulence by an in vitro glass adherence test.
作者信息
Solano C, Sesma B, Alvarez M, Humphrey T J, Thorns C J, Gamazo C
机构信息
Departamento de Microbiología, Universidad de Navarra, Pamplona, Spain.
出版信息
J Clin Microbiol. 1998 Mar;36(3):674-8. doi: 10.1128/JCM.36.3.674-678.1998.
Abstract
The objective of this study was the in vitro differentiation of isolates of Salmonella enteritidis whose virulences differed in a chick model. A total of 14 strains of S. enteritidis were isolated from either the environment, dairy products, or infected patients. The isolates could be divided into two groups on the basis of their virulence (50% lethal dose) in chickens infected intraperitoneally. When the strains were incubated in adherence test medium (Spanish patent 9700408), only the virulent strains produced aggregates and formed visible filaments attached to the glass tube. These results suggest, although for a limited number of strains, that aggregation in such a medium could be used as a diagnostic tool to discriminate virulent strains of S. enteritidis.
摘要
本研究的目的是对肠炎沙门氏菌分离株进行体外分化，这些分离株在雏鸡模型中的毒力有所不同。总共从环境、乳制品或感染患者中分离出14株肠炎沙门氏菌。根据这些分离株对腹腔注射感染的鸡的毒力（半数致死剂量），可将它们分为两组。当这些菌株在黏附测试培养基（西班牙专利9700408）中培养时，只有有毒力的菌株会产生聚集体，并形成附着在玻璃管上的可见细丝。这些结果表明，尽管菌株数量有限，但在这种培养基中的聚集现象可作为一种诊断工具，用于区分肠炎沙门氏菌的有毒力菌株。
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