基于rRNA基因内部转录间隔区核苷酸序列变异的人肺孢子菌分型研究进展
Update on Pneumocystis carinii f. sp. hominis typing based on nucleotide sequence variations in internal transcribed spacer regions of rRNA genes.
作者信息
Lee C H, Helweg-Larsen J, Tang X, Jin S, Li B, Bartlett M S, Lu J J, Lundgren B, Lundgren J D, Olsson M, Lucas S B, Roux P, Cargnel A, Atzori C, Matos O, Smith J W
机构信息
Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis 46202, USA.
出版信息
J Clin Microbiol. 1998 Mar;36(3):734-41. doi: 10.1128/JCM.36.3.734-741.1998.
Abstract
Pneumocystis carinii f. sp. hominis isolates from 207 clinical specimens from nine countries were typed based on nucleotide sequence variations in the internal transcribed spacer regions I and II (ITS1 and ITS2, respectively) of rRNA genes. The number of ITS1 nucleotides has been revised from the previously reported 157 bp to 161 bp. Likewise, the number of ITS2 nucleotides has been changed from 177 to 192 bp. The number of ITS1 sequence types has increased from 2 to 15, and that of ITS2 has increased from 3 to 14. The 15 ITS1 sequence types are designated types A through O, and the 14 ITS2 types are named types a through n. A total of 59 types of P. carinii f. sp. hominis were found in this study.
摘要
基于rRNA基因内部转录间隔区I和II（分别为ITS1和ITS2）的核苷酸序列变异，对来自9个国家的207份临床标本中的人肺孢子菌进行了分型。ITS1核苷酸的数量已从先前报道的157 bp修订为161 bp。同样，ITS2核苷酸的数量已从177 bp变为192 bp。ITS1序列类型的数量从2种增加到15种，ITS2的数量从3种增加到14种。15种ITS1序列类型被指定为A至O型，14种ITS2类型被命名为a至n型。本研究共发现59种人肺孢子菌。
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