Chorvatovicová D, Machová E, Sandula J
Institute of Experimental Pharmacology, Slovak Academy of Sciences, Bratislava, Slovak Republic.
Mutat Res. 1998 Jan 13;412(1):83-9. doi: 10.1016/s1383-5718(97)00176-9.
Carboxymethyl-chitin-glucan (CMCG) isolated from Aspergillus niger was ultrasonicated to decrease its molecular weight. Ultrasonicated CMCG with molecular weight 0.19 x 10(-5) was administered either intraperitoneally or orally prior to cyclophosphamide (CP) injection and its effect on the frequency of micronuclei in polychromatic erythrocytes of mouse bone marrow was evaluated. Both ways of CMCG administration significantly decreased the clastogenic effect of CP. The protective effect of CMCG was concentration dependent, with a higher decrease achieved by 200 mg/kg than by 100 mg/kg b.wt. Ultrasonic depolymerization of high molecular CMCG resulted in its anticlastogenic effect against CP not only on intraperitoneal, but also on oral administration, achieved by decreasing its molecular weight. Ultrasonication proved to be an efficient way to obtain molecules of CMCG able to pass through the cell walls of the gastrointestinal tract.
从黑曲霉中分离得到的羧甲基几丁质-葡聚糖（CMCG）经超声处理以降低其分子量。将分子量为0.19×10⁻⁵的超声处理后的CMCG在注射环磷酰胺（CP）之前经腹腔或口服给药，并评估其对小鼠骨髓多染红细胞中微核频率的影响。两种CMCG给药方式均显著降低了CP的致断裂效应。CMCG的保护作用呈浓度依赖性，200mg/kg体重组比100mg/kg体重组的降低幅度更大。高分子量CMCG的超声解聚不仅通过腹腔给药，而且通过口服给药，都使其对CP产生抗断裂效应，这是通过降低其分子量实现的。事实证明，超声处理是获得能够穿过胃肠道细胞壁的CMCG分子的有效方法。
