Chorvatovicová D, Machová E, Sandula J
Institute of Experimental Pharmacology Slovak Academy of Sciences, Bratislava, Slovak Republic.
Mutat Res. 1996 Nov 4;371(1-2):115-20. doi: 10.1016/s0165-1218(96)90101-9.
Carboxymethylglucan (CMG) with ultrasonically lowered molecular weight (0.89 x 10(5)) was administered either intraperitoneally, intravenously or orally prior to cyclophosphamide (CP) injection and its effect on the frequency of micronuclei in mouse bone marrow was evaluated. Both parenteral (intraperitoneal and intravenous) and oral administration of CMG decreased the clastogenic effect of CP. The protective effect induced by intravenous and intraperitoneal administration was concentration-dependent, with a higher decrease achieved by 200 mg/kg than by 100 mg/kg body weight. With the lower dose of CMG a 2-h interval was necessary between intravenous CMG administration and CP injection. Oral pretreatment of mice with CMG decreased statistically significantly the frequency of micronuclei in polychromatic erythrocytes of the bone marrow. The fact that ultrasonically depolymerized CMG was effective also on oral administration is indicative of the passage of smaller CMG molecules through the wall of the gastrointestinal tract.
在注射环磷酰胺（CP）之前，腹腔内、静脉内或口服给予经超声处理分子量降低（0.89×10⁵）的羧甲基葡聚糖（CMG），并评估其对小鼠骨髓微核频率的影响。CMG的肠胃外（腹腔内和静脉内）给药及口服给药均降低了CP的致断裂效应。静脉内和腹腔内给药诱导的保护作用呈浓度依赖性，体重200mg/kg比100mg/kg产生的降低幅度更大。使用较低剂量的CMG时，静脉内给予CMG与注射CP之间需要间隔2小时。用CMG对小鼠进行口服预处理可使骨髓中多色红细胞的微核频率在统计学上显著降低。经超声解聚的CMG口服也有效，这表明较小的CMG分子可穿过胃肠道壁。
