通过PCR-SHELA和巢式PCR检测口咽冲洗液中的卡氏肺孢子菌。
Detection of Pneumocystis carinii in oropharyngeal washings by PCR-SHELA and nested PCR.
作者信息
Tamburrini E, Ortona E, Visconti E, Margutti P, Mencarini P, Zolfo M, Marinaci S, Siracusano A
机构信息
Dept. Infectious Diseases, Università Cattolica S. Cuore, Rome, Italy.
出版信息
J Eukaryot Microbiol. 1997 Nov-Dec;44(6):48S. doi: 10.1111/j.1550-7408.1997.tb05769.x.
Oropharyngeal washings (Ophs) from 27 HIV infected patients (18 with P. carinii pneumonia, PCP, and 9 without PCP) were examined for P. carinii using morphological staining and DNA amplification with PCR-SHELA and nested PCR methods. The comparison of these techniques shows that 1. the amplification of P. carinii DNA is more sensitive than (and as specific as) morphological staining; 2. PCR-SHELA is less sensitive than (and as specific as) nested PCR.
对27例HIV感染患者（18例患有卡氏肺孢子虫肺炎，9例未患卡氏肺孢子虫肺炎）的口咽冲洗液进行检测，采用形态学染色以及PCR-SHELA和巢式PCR方法进行DNA扩增，以检测其中的卡氏肺孢子虫。这些技术的比较表明：1. 卡氏肺孢子虫DNA扩增比形态学染色更敏感（特异性相同）；2. PCR-SHELA比巢式PCR敏感性更低（特异性相同）。
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