Colombo P, Kennedy D, Ramsdale T, Costa M A, Duro G, Izzo V, Salvadori S, Guerrini R, Cocchiara R, Mirisola M G, Wood S, Geraci D
Istituto di Biologia dello Sviluppo Consiglio Nazionale Delle Ricerche, Palermo, Italy.
J Immunol. 1998 Mar 15;160(6):2780-5.
Par j 1.0101 is one of the two major allergens of the Parietaria judaica (Pj) pollen, and its three-dimensional structure was built by three-dimensional structural homology modeling. The resultant model was used to identify putative IgE binding regions. Western blot analysis of gene fragmentation products showed that the 1 to 30 region was capable of binding specific IgE from a pool of sera (n = 30) of patients allergic to Pj pollen. Using the structural model as a guide, deletion and site-directed mutagenesis of the 1 to 30 region was performed, and the amino acids involved in IgE binding were identified. In addition, a synthetic peptide covering the 1 to 30 region was capable of binding human IgE without triggering histamine release from basophils of Pj allergic patients (n = 6) and thus represents a haptenic molecule with potential use as an immunotolerant agent. This epitope is also present on the Par j 2.0101 major allergen representing a common IgE epitope. It is an immunodominant epitope, since it was capable of inhibiting 30% of all specific IgE against the Pj major allergens, and therefore, it might be a candidate for the future development of immunotherapeutics.
Par j 1.0101是墙草（Pj）花粉的两种主要过敏原之一，其三维结构通过三维结构同源性建模构建。所得模型用于识别假定的IgE结合区域。基因片段产物的蛋白质印迹分析表明，1至30区域能够结合来自对Pj花粉过敏患者的一组血清（n = 30）中的特异性IgE。以结构模型为指导，对1至30区域进行缺失和定点诱变，并鉴定参与IgE结合的氨基酸。此外，覆盖1至30区域的合成肽能够结合人IgE，而不会触发来自Pj过敏患者（n = 6）嗜碱性粒细胞的组胺释放，因此代表一种具有潜在用作免疫耐受剂的半抗原分子。该表位也存在于代表常见IgE表位的Par j 2.0101主要过敏原上。它是一个免疫显性表位，因为它能够抑制30%的针对Pj主要过敏原的所有特异性IgE，因此，它可能是未来免疫治疗发展的候选物。
