Kim J H, Ko M K, Shin J C
Department of Ophthalmology, School of Medicine, Hanyang University, Seoul, Korea.
Korean J Ophthalmol. 1997 Dec;11(2):84-8. doi: 10.3341/kjo.1997.11.2.84.
In patients with herpes virus dendritic epithelial keratitis, the pathologic morphogenesis of the dendritic lesion was investigated by means of impression cytology. Impression specimens were taken from the lesions of 24 consecutive patients assessed with a slit lamp biomicroscope. After a Millipore membrane impression was stained with hematoxylin-eosin and examined by light microscope, cellular patterns were analyzed. The results of these examinations showed that most cells were epithelial cells; there were also occasional multinucleated syncytial giant cells, inclusion bodies and inflammatory cells. Basal epithelial cells were most prominent at the actively infected lesion, whereas superficial epithelial cells were shown at the margin of the active lesion and terminal bulb. These findings suggest that in dendritic keratitis, viral spread and replication in the dendritic keratitis may progress through basal epithelial cells along the nerve within the basal epithelium-stromal interface.
在疱疹病毒性树枝状上皮角膜炎患者中，通过印片细胞学方法研究了树枝状病变的病理形态发生。用裂隙灯生物显微镜对24例连续患者的病变部位进行取材，制作印片标本。将微孔滤膜印片用苏木精-伊红染色后，用光镜检查并分析细胞形态。这些检查结果显示，大多数细胞为上皮细胞；也偶尔可见多核的合胞体巨细胞、包涵体和炎性细胞。在活跃感染的病变部位，基底上皮细胞最为突出，而在活跃病变的边缘和终末球可见表层上皮细胞。这些发现提示，在树枝状角膜炎中，病毒在树枝状角膜炎中的传播和复制可能沿着基底上皮-基质界面内的神经通过基底上皮细胞进行。
