Sugimoto M, Sakamoto W
Laboratory of Biochemistry, Okayama University, Japan.
Genes Genet Syst. 1997 Oct;72(5):311-6. doi: 10.1266/ggs.72.311.
An Arabidopsis cDNA encoding putative phospholipid hydroperoxide glutathione peroxidase (PHGPX) was cloned and sequenced. The cDNA comprised 803 bp and included an open reading frame which encodes a polypeptide of 169 amino acid residues. The deduced amino acid sequence showed about 80 and 50% homology with plant putative PHGPXs and mammalian PHGPXs, respectively. Southern blot analysis suggested that putative PHGPX gene was a single-copy gene. The expression profile of the putative PHGPX in Arabidopsis under NaCl and Al/Fe treatments, which generate oxidative stress, was analyzed. Northern blot analysis revealed that the Arabidopsis putative PHGPX mRNA levels were increased about 3 and 4.5 times after exposure to NaCl and Al/Fe, respectively. These results suggest that the putative PHGPX gene is induced by oxidative stress in Arabidopsis.
克隆并测序了拟南芥中一个编码假定磷脂氢过氧化物谷胱甘肽过氧化物酶（PHGPX）的cDNA。该cDNA由803个碱基对组成，包含一个开放阅读框，编码一个由169个氨基酸残基组成的多肽。推导的氨基酸序列与植物假定的PHGPX和哺乳动物PHGPX的同源性分别约为80%和50%。Southern杂交分析表明假定的PHGPX基因是单拷贝基因。分析了拟南芥中假定的PHGPX在产生氧化应激的NaCl和Al/Fe处理下的表达谱。Northern杂交分析显示，分别暴露于NaCl和Al/Fe后，拟南芥假定的PHGPX mRNA水平分别增加了约3倍和4.5倍。这些结果表明，拟南芥中假定的PHGPX基因受氧化应激诱导。
