Hawser S P, Baillie G S, Douglas L J
Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of Glasgow.
J Med Microbiol. 1998 Mar;47(3):253-6. doi: 10.1099/00222615-47-3-253.
Growth of Candida albicans biofilms and production of extracellular matrix were monitored by dry weight, colorimetric and radioisotope assays, and by scanning electron microscopy. Under static incubation conditions synthesis of matrix material was minimal, but increased dramatically when developing biofilms were subjected to a liquid flow with the result that the cells were enveloped in extracellular polymer. These findings suggest that production of matrix material could contribute to the resistance of biofilm cells to antifungal agents in vivo.
通过干重法、比色法和放射性同位素测定法以及扫描电子显微镜对白色念珠菌生物膜的生长和细胞外基质的产生进行了监测。在静态培养条件下，基质材料的合成极少，但当发育中的生物膜受到液体流动作用时，合成量急剧增加，结果细胞被细胞外聚合物包裹。这些发现表明，基质材料的产生可能有助于生物膜细胞在体内对抗真菌剂的抗性。
