RNA聚合酶中的力产生
Force generation in RNA polymerase.
作者信息
Wang H Y, Elston T, Mogilner A, Oster G
机构信息
Department of Molecular and Cellular Biology, University of California, Berkeley 94720-3112, USA.
出版信息
Biophys J. 1998 Mar;74(3):1186-202. doi: 10.1016/S0006-3495(98)77834-8.
Abstract
RNA polymerase (RNAP) is a processive molecular motor capable of generating forces of 25-30 pN, far in excess of any other known ATPase. This force derives from the hydrolysis free energy of nucleotides as they are incorporated into the growing RNA chain. The velocity of procession is limited by the rate of pyrophosphate release. Here we demonstrate how nucleotide triphosphate binding free energy can rectify the diffusion of RNAP, and show that this is sufficient to account for the quantitative features of the measured load-velocity curve. Predictions are made for the effect of changing pyrophosphate and nucleotide concentrations and for the statistical behavior of the system.
摘要
RNA聚合酶（RNAP）是一种持续性分子马达，能够产生25-30皮牛的力，远远超过任何其他已知的ATP酶。这种力源于核苷酸水解自由能，当它们被掺入不断增长的RNA链中时。行进速度受焦磷酸释放速率的限制。在这里，我们展示了三磷酸核苷酸结合自由能如何纠正RNAP的扩散，并表明这足以解释测量的负载-速度曲线的定量特征。对改变焦磷酸和核苷酸浓度的影响以及系统的统计行为进行了预测。
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