Diogo M M, Cabral J M, Queiroz J A
Departamento de Química, Universidade da Beira Interior, Covilhã, Portugal.
J Chromatogr A. 1998 Feb 13;796(1):177-80. doi: 10.1016/s0021-9673(97)01059-5.
The purification of Chromobacterium viscosum lipase was performed using a polypropylene glycol-Sepharose gel. The influence of the mobile phase composition on the chromatographic behaviour of Chromobacterium viscosum lipase was studied and it was found that the retention of lipase depends on the salt used and increased with ionic strength. Using 20% (w/v) ammonium sulphate in the eluent, a total retention of lipase on the column was obtained.
使用聚丙二醇-琼脂糖凝胶对粘质沙雷氏菌脂肪酶进行纯化。研究了流动相组成对粘质沙雷氏菌脂肪酶色谱行为的影响，发现脂肪酶的保留率取决于所用盐类，并随离子强度的增加而提高。在洗脱液中使用20%（w/v）硫酸铵时，脂肪酶在柱上实现了完全保留。
