Horiuti Y, Koga H, Gocho S
J Biochem. 1976 Aug;80(2):367-70. doi: 10.1093/oxfordjournals.jbchem.a131285.
A method was devised for activity assay of the lipase [triacylglycerol acyl-hydrolase, EC 3.1.1.3] excreted from Chromobacterium viscosum into the culture medium; olive oil emulsified with the aid of Adekatol 45-S-8 (a non-ionic detergent, the ethoxylate of linear sec-alcohols having chain lengths of 10--16 carbon atoms) was used as the substrate. This method was specifically effective for Chromobacterium lipase acitvity assay, and was approximately twice as sensitive as the conventional method, in which polyvinyl alcohol is used for the emulsification of the substrate.
设计了一种用于测定从粘性色杆菌分泌到培养基中的脂肪酶[三酰基甘油酰基水解酶，EC 3.1.1.3]活性的方法；以借助于Adekatol 45-S-8（一种非离子洗涤剂，链长为10 - 16个碳原子的直链仲醇的乙氧基化物）乳化的橄榄油作为底物。该方法对粘性色杆菌脂肪酶活性测定具有特异性效果，并且比使用聚乙烯醇乳化底物的传统方法灵敏度高约两倍。
