Yamasaki M, Miyake A, Tagashira S, Itoh N
Department of Genetic Biochemistry, Kyoto University Faculty of Pharmaceutical Sciences, Kyoto 606-01, Japan.
J Biol Chem. 1996 Jul 5;271(27):15918-21. doi: 10.1074/jbc.271.27.15918.
We isolated the cDNA encoding a novel member of the fibroblast growth factor (FGF) family from rat embryos by homology-based polymerase chain reaction. The FGF-related cDNA encodes a protein of 215 amino acids (approximately 24 kDa), which has a conserved approximately 120-amino acid core with approximately 30-60% amino acid sequence identity with the FGF family. This protein with a hydrophobic amino terminus appears to be a secreted protein. The cDNA was translated in a coupled in vitro transcription-translation system. The molecular mass of the translation product was observed to be approximately 26 kDa. The expression of the FGF-related mRNA in the rat embryo and adult tissues was determined by Northern analysis and in situ hybridization. The mRNA was expressed in several discrete regions of the embryo. In adult tissues, the mRNA was preferentially expressed in the lung. The expression profile of the FGF-related mRNA was different from those of other FGF family mRNAs. As this protein is the 10th documented protein related to FGFs, we tentatively term this protein FGF-10.
我们通过基于同源性的聚合酶链反应从大鼠胚胎中分离出编码成纤维细胞生长因子（FGF）家族一个新成员的cDNA。该FGF相关的cDNA编码一个由215个氨基酸组成的蛋白质（约24 kDa），它有一个保守的约120个氨基酸的核心区域，与FGF家族的氨基酸序列一致性约为30%-60%。这个具有疏水氨基末端的蛋白质似乎是一种分泌蛋白。该cDNA在一个体外转录-翻译偶联系统中进行翻译。观察到翻译产物的分子量约为26 kDa。通过Northern分析和原位杂交确定了FGF相关mRNA在大鼠胚胎和成年组织中的表达情况。该mRNA在胚胎的几个离散区域表达。在成年组织中，该mRNA优先在肺中表达。FGF相关mRNA的表达谱与其他FGF家族mRNA的表达谱不同。由于这种蛋白质是第10个被记录的与FGF相关的蛋白质，我们暂时将这种蛋白质命名为FGF-10。
