Kushida N, Atsumi S, Koyano T, Umezawa K
Department of Applied Chemistry, Faculty of Science and Technology, Keio University, Yokohama, Japan.
Drugs Exp Clin Res. 1997;23(5-6):151-5.
In the course of screening for Ras function inhibitors from plant extracts, we isolated lycorine from a chloroform extract of Eucharis grandiflora leaves. Lycorine induced flat morphology in K-ras-NRK cells after treatment for 2-3 days, whereas its morphological effect on NRK cells was weaker. Lycorine was found to inhibit protein synthesis specifically in cultured K-ras-NRK cells. It also lowered the cellular amount of Ras in 2-3 days.
在从植物提取物中筛选Ras功能抑制剂的过程中，我们从大花油加律叶的氯仿提取物中分离出了石蒜碱。处理2 - 3天后，石蒜碱在K-ras-NRK细胞中诱导出扁平形态，而其对NRK细胞的形态学影响较弱。发现石蒜碱特异性抑制培养的K-ras-NRK细胞中的蛋白质合成。它还在2 - 3天内降低了细胞内Ras的含量。
