Ouellet F, Vazquez-Tello A, Sarhan F
Département des Sciences biologiques, Université du Québec à Montréal, Canada.
FEBS Lett. 1998 Feb 27;423(3):324-8. doi: 10.1016/s0014-5793(98)00116-1.
The wcs120 gene is specifically induced by low temperature (LT) and encodes a protein that is thought to play an important role in the cold acclimation process in wheat. To identify the regulatory elements involved in its LT responsiveness, the transient expression activity of different promoter regions was determined using the luciferase reporter gene. The data indicate the involvement of putative enhancer elements, negative and positive regulatory regions in the transcriptional regulation of this gene. The promoter was found to be cold-inducible in different freezing-tolerant and -sensitive monocot and dicot species, suggesting that universal transcription factors responsive to LT may be present in all plants. This promoter could be used to drive the genes needed for LT tolerance in sensitive species.
wcs120基因受低温（LT）特异性诱导，编码一种被认为在小麦冷驯化过程中起重要作用的蛋白质。为了鉴定其低温响应中涉及的调控元件，使用荧光素酶报告基因测定了不同启动子区域的瞬时表达活性。数据表明，推定的增强子元件、负调控区和正调控区参与了该基因的转录调控。发现该启动子在不同的耐冻和冻敏感单子叶和双子叶物种中可被冷诱导，这表明对低温有响应的通用转录因子可能存在于所有植物中。该启动子可用于驱动敏感物种中耐低温所需的基因。
