低温胁迫下钼对冬小麦脱落酸（ABA）依赖和ABA非依赖途径中冷响应基因表达的影响
Effects of molybdenum on expression of cold-responsive genes in abscisic acid (ABA)-dependent and ABA-independent pathways in winter wheat under low-temperature stress.
作者信息
Sun Xuecheng, Hu Chengxiao, Tan Qilin, Liu Jinshan, Liu Hongen
机构信息
Key Laboratory of Subtropical Agriculture and Environment, Ministry of Agriculture, Wuhan, China.
出版信息
Ann Bot. 2009 Aug;104(2):345-56. doi: 10.1093/aob/mcp133. Epub 2009 Jun 1.
BACKGROUND AND AIMS
Molybdenum (Mo) is an essential trace element for higher plants. It has been shown that application of Mo enhances the cold resistance of winter wheat. In order to improve our understanding of the molecular mechanisms of cold resistance arising from application of Mo in winter wheat, investigations were made regarding the transcription of cold-responsive (COR) genes in abscisic acid (ABA)-dependent and ABA-independent pathways in winter wheat regulated by Mo application under low-temperature stress.
METHODS
Two cultivars of winter wheat (Triticum aestivum), Mo-efficient cultivar '97003' and Mo-inefficient cultivar '97014', were grown in control (-Mo) and Mo fertilizer (+Mo) treatments for 40 d at 15/12 degrees C (day/night), and the temperature was then reduced to 5/2 degrees C (day/night) to create low-temperature stress. Aldehyde oxidase (AO) activities, ABA contents, the transcripts of basic leucine zipper (bZIP)-type transcription factor (TF) genes, ABA-dependent COR genes, CBF/DREB transcription factor genes and ABA-independent COR genes were investigated at 0, 3, 6 and 48 h post cold stress.
KEY RESULTS
Mo application significantly increased AO activity, ABA levels, and expression of bZIP-type TF genes (Wlip19 and Wabi5) and ABA-dependent COR genes (Wrab15, Wrab17, Wrab18 and Wrab19). Mo application increased expression levels of CBF/DREB transcription factor genes (TaCBF and Wcbf2-1) and ABA-independent COR genes (Wcs120, Wcs19, Wcor14 and Wcor15) after 3 and 6 h exposure to low temperature.
CONCLUSIONS
Mo might regulate the expression of ABA-dependent COR genes through the pathway: Mo --> AO --> ABA --> bZIP --> ABA-dependent COR genes in winter wheat. The response of the ABA-dependent pathway to Mo was prior to that of the ABA-independent pathway. Similarities and differences between the Mo-efficient and Mo-inefficient wheat cultivars in response to Mo under cold stress are discussed.
背景与目的
钼（Mo）是高等植物必需的微量元素。研究表明，施用钼可增强冬小麦的抗寒性。为了深入了解冬小麦施用钼后抗寒的分子机制，本研究对低温胁迫下钼调控的冬小麦脱落酸（ABA）依赖和ABA非依赖途径中冷响应（COR）基因的转录情况进行了研究。
方法
选用两个冬小麦（普通小麦）品种，钼高效品种‘97003’和钼低效品种‘97014’，在15/12℃（昼/夜）条件下进行对照（-Mo）和施钼（+Mo）处理40天，然后将温度降至5/2℃（昼/夜）以产生低温胁迫。在冷胁迫后0、3、6和48小时，检测醛氧化酶（AO）活性、ABA含量、碱性亮氨酸拉链（bZIP）型转录因子（TF）基因、ABA依赖的COR基因、CBF/DREB转录因子基因和ABA非依赖的COR基因的转录本。
关键结果
施用钼显著提高了AO活性、ABA水平以及bZIP型TF基因（Wlip19和Wabi5）和ABA依赖的COR基因（Wrab15、Wrab17、Wrab18和Wrab19）的表达。低温处理3和6小时后，施用钼提高了CBF/DREB转录因子基因（TaCBF和Wcbf2-1）和ABA非依赖的COR基因（Wcs120、Wcs19、Wcor14和Wcor15）的表达水平。
结论
钼可能通过以下途径调控冬小麦ABA依赖的COR基因表达：钼→AO→ABA→bZIP→ABA依赖的COR基因。ABA依赖途径对钼的响应先于ABA非依赖途径。讨论了钼高效和钼低效小麦品种在冷胁迫下对钼响应的异同。
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