两种新型链霉菌核糖核酸酶抑制剂SaI14和SaI20的分离与纯化，以及编码SaI14的基因在大肠杆菌中的克隆、测序和表达。
Isolation and purification of two novel streptomycete RNase inhibitors, SaI14 and SaI20, and cloning, sequencing, and expression in Escherichia coli of the gene coding for SaI14.
作者信息
Krajcikova D, Hartley R W, Sevcik J
机构信息
Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, Slovakia.
出版信息
J Bacteriol. 1998 Mar;180(6):1582-5. doi: 10.1128/JB.180.6.1582-1585.1998.
Abstract
Two new RNase inhibitors, SaI14 (Mr, approximately 14,000) and SaI20 (Mr, approximately 20,000), were isolated and purified from a Streptomyces aureofaciens strain. The gene sai14, coding for SaI14 protein, was cloned and expressed in Escherichia coli. The alignment of the deduced amino acid sequence of SaI14 with that of barstar, the RNase inhibitor from Bacillus amyloliquefaciens, showed significant similarity between them, especially in the region which contains most of the residues involved in barnase-barstar complex formation.
摘要
从一株金色链霉菌中分离并纯化出两种新的核糖核酸酶抑制剂，即SaI14（分子量约为14,000）和SaI20（分子量约为20,000）。编码SaI14蛋白的基因sai14被克隆并在大肠杆菌中表达。SaI14推导的氨基酸序列与解淀粉芽孢杆菌的核糖核酸酶抑制剂barstar的氨基酸序列比对显示，它们之间存在显著相似性，尤其是在包含参与barnase-barstar复合物形成的大多数残基的区域。
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