Der-Silaphet T, Malysz J, Hagel S, Larry Arsenault A, Huizinga J D
Intestinal Disease Research Programme and Department of Biomedical Sciences, McMaster University, Hamilton, Ontario, Canada.
Gastroenterology. 1998 Apr;114(4):724-36. doi: 10.1016/s0016-5085(98)70586-4.
BACKGROUND & AIMS: Interstitial cells of Cajal (ICC) have been linked to the generation of intestinal pacemaker activity, but their role in in vivo motor dysfunction is unclear. In this study, we investigated the hypothesis that ICC play a role in the generation of distention-induced peristalsis using W/Wv mice that lack ICC associated with Auerbach's plexus.
Radiological observations were made of the movement of contrast fluid through the proximal small intestine. Electrical activities were recorded in the external muscle layers. In addition, intraluminal pressure changes were recorded in isolated intestinal segments.
In control mice, after gavage of 0.5 mL of barium sulfate in the stomach, the contrast fluid moved through the proximal small intestine in peristaltic waves at approximately 47 times a minute, propagating aborally at approximately 2 cm/s. Electrical slow waves and intraluminal pressure waves were synchronized at similar frequencies and propagation velocities. In W/Wv mice, such regular peristaltic waves were not observed. Action potentials and contractions appeared random, and contents moved back and forth in an irregular manner. The net propulsive effect of contractile activity in W/Wv mutant mice was much weaker than that in controls.
Slow wave controlled peristalsis occurs in the normal proximal small intestine upon gastric emptying of a semiliquid. This motor pattern is absent in W/Wv mice that lack ICC.
Cajal间质细胞（ICC）与肠道起搏活动的产生有关，但其在体内运动功能障碍中的作用尚不清楚。在本研究中，我们使用缺乏与奥尔巴赫神经丛相关的ICC的W/Wv小鼠，研究ICC在扩张诱导的蠕动产生中发挥作用的假说。
对造影剂在近端小肠中的移动进行放射学观察。记录外层肌肉的电活动。此外，在离体肠段记录腔内压力变化。
在对照小鼠中，胃内灌胃0.5 mL硫酸钡后，造影剂以每分钟约47次的蠕动波通过近端小肠，以约2 cm/s的速度向口外传播。电慢波和腔内压力波在相似的频率和传播速度上同步。在W/Wv小鼠中，未观察到这种规则的蠕动波。动作电位和收缩显得随机，内容物以不规则的方式来回移动。W/Wv突变小鼠收缩活动的净推进作用比对照小鼠弱得多。
半液体胃排空后，正常近端小肠会出现慢波控制的蠕动。这种运动模式在缺乏ICC的W/Wv小鼠中不存在。
