Perri F, Clemente R, Pastore M, Quitadamo M, Festa V, Bisceglia M, Li Bergoli M, Lauriola G, Leandro G, Ghoos Y, Rutgeerts P, Andriulli A
Department of Human Pathology, Casa Sollievo della Sofferenza Hospital, IRCCS, San Giovanni Rotondo, Italy.
Scand J Clin Lab Invest. 1998 Feb;58(1):19-27. doi: 10.1080/00365519850186797.
The urea breath test (UBT) has been proposed as the most accurate test for diagnosing Helicobacter pylori infection. The aim of this work was to evaluate the accuracy of the UBT and to compare the results with histologic and endoscopic findings in H. pylori infected patients.
One-hundred-and-seventy-two consecutive dyspeptic outpatients were studied by means of endoscopy (with histology and culture), UBT (75 mg 13C-urea), and serology. Gastritis was classified in accordance with the Sydney criteria. In H. pylori positive patients, the bacterial load was assessed semiquantitatively, the number of bacteria in histologic specimens being counted. UBT results were expressed either as percentage cumulative dose of 13CO2 excreted at 1 h (CD60) or delta over baseline at 30' (DOB30).
Of 172 patients, 126 (73%) were H. pylori positive on histology or culture. Using a cut-off value of 3.3/1000 for DOB30, the sensitivity, specificity and accuracy of the UBT were 96%, 93.5%, and 95.3%, respectively. A significant correlation was observed between DOB30 values and intragastric bacterial load (r = 0.32). Moreover, a significant difference in DOB30 values was found between patients sorted by the depth of inflammation (chi(2) = 4.36, p = 0.036). No correlation was observed between DOB30 and endoscopic findings in H. pylori positive subjects.
The UBT is an accurate non-invasive diagnostic tool and can be used to predict both the intragastric bacterial load and the severity of related gastritis.
尿素呼气试验（UBT）已被认为是诊断幽门螺杆菌感染最准确的检测方法。本研究的目的是评估UBT的准确性，并将其结果与幽门螺杆菌感染患者的组织学和内镜检查结果进行比较。
对172例连续性消化不良门诊患者进行了内镜检查（包括组织学检查和培养）、UBT（75mg 13C-尿素）和血清学检查。根据悉尼标准对胃炎进行分类。在幽门螺杆菌阳性患者中，对细菌载量进行半定量评估，计算组织学标本中的细菌数量。UBT结果以1小时时呼出的13CO2累积剂量百分比（CD60）或30分钟时相对于基线的变化量（DOB30）表示。
172例患者中，126例（73%）组织学或培养结果显示幽门螺杆菌阳性。以DOB30的截断值为3.3/1000时，UBT的敏感性、特异性和准确性分别为96%、93.5%和95.3%。观察到DOB30值与胃内细菌载量之间存在显著相关性（r = 0.32）。此外，根据炎症深度分类的患者之间DOB30值存在显著差异（χ2 = 4.36，p = 0.036）。在幽门螺杆菌阳性受试者中，未观察到DOB30与内镜检查结果之间的相关性。
UBT是一种准确的非侵入性诊断工具，可用于预测胃内细菌载量和相关胃炎的严重程度。
