Murphy C R, Swift J G, Mukherjee T M, Rogers A W
Cell Biophys. 1979 Jun;1(2):181-93. doi: 10.1007/BF02781350.
Freeze fracture techniques have been used to study the apical membrane of cells of the luminal epithelium of the rat uterus under various hormonal regimes. In the ovariectomized but otherwise untreated rat, intramembranous particles (IMPs) occur at a density of 1395 +/- 122 per micron 2; they appeared spherical and uniformly distributed. After 3 days treatment with estrogen, no change in appearance or density was found, but 3 days of progesterone treatment produced a significant increase in IMP density to 1622 +/- 104. Treatment with progesterone, with an additional dose of estrogen on day 3, is known to produce an epithelium receptive to the implanting blastocyst. In these conditions, the IMP density rose to 3818 +/- 337: rod-shaped particles and aggregations of IMPs were seen, and some particle arrays resembling gap junctions, in addition to the isolated spherical particles.
冷冻断裂技术已被用于研究在不同激素状态下大鼠子宫腔上皮细胞顶端膜。在去卵巢但未接受其他处理的大鼠中，膜内颗粒（IMPs）以每平方微米1395±122的密度出现；它们呈球形且分布均匀。用雌激素处理3天后，外观或密度未发现变化，但用孕酮处理3天导致IMP密度显著增加至1622±104。已知在第3天用孕酮并额外加一剂雌激素处理可使上皮对植入的胚泡具有接受性。在这些条件下，IMP密度升至3818±337：可见杆状颗粒和IMP聚集，除了孤立的球形颗粒外，还可见一些类似缝隙连接的颗粒阵列。
