Hiramoto K, Tomiyama S, Kikugawa K
School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Japan.
Free Radic Res. 1997 Nov;27(5):505-9. doi: 10.3109/10715769709065790.
Whether dithiocarbamate-Fe(II) complexes used in the electron spin resonance (ESR) studies are specific for the detection of NO was investigated. It was found that the typical ESR signals of dithiocarbamate-Fe(II)-NO spin adducts appeared in the interaction of N-methyl-D-glucaminedithiocarbamate (MGD)-, bis(hydroxyethyl) dithiocarbamate (HED)- and diethyldithiocarbamate (DED)-Fe(II) complexes with the NO-derived nitrogen oxides, nitrogen dioxide (NO2) and nitrite ion (NO2-) in a prolonged incubation. Under the conditions of prolonged incubation, appearance of the ESR signals of the dithiocarbamate-Fe(II)-NO spin adducts may indicate the presence of not only NO but NO2 and NO2-.
研究了电子自旋共振（ESR）研究中使用的二硫代氨基甲酸盐 - 铁（II）配合物是否对一氧化氮（NO）的检测具有特异性。结果发现，在长时间孵育过程中，N - 甲基 - D - 葡糖胺二硫代氨基甲酸盐（MGD）、双（羟乙基）二硫代氨基甲酸盐（HED）和二乙基二硫代氨基甲酸盐（DED） - 铁（II）配合物与源自NO的氮氧化物、二氧化氮（NO₂）和亚硝酸根离子（NO₂⁻）相互作用时，出现了二硫代氨基甲酸盐 - 铁（II） - NO自旋加合物的典型ESR信号。在长时间孵育条件下，二硫代氨基甲酸盐 - 铁（II） - NO自旋加合物ESR信号的出现可能不仅表明存在NO，还表明存在NO₂和NO₂⁻。
