Soumano K, Lussier J G, Price C A
Centre de Recherche en Reproduction Animale, Faculté de Médecine Véterinaire, Université de Montréal, Quebec, Canada.
J Endocrinol. 1998 Feb;156(2):373-8. doi: 10.1677/joe.0.1560373.
This study tested the hypothesis that luteal LH receptor (LHr) and follicular LHr and FSH receptor (FSHr) steady-state mRNA levels are greater during superovulation with equine chorionic gonadotrophin (eCG) compared with that with FSH. Heifers were stimulated with eCG (n = 10) or FSH (n = 10), and ovaries were recovered the day before and at 12 and 24 h after luteolysis was induced with prostaglandin F2 alpha (PGF2 alpha). Total RNA was purified from individual follicles and corpora lutea. Steady-state levels of LHr and FSHr mRNA were assessed by slot blot analysis employing homologous cDNA probes. There were no differences in luteal LHr between FSH- and eCG-stimulated animals before luteolysis, and hybridization signals were detected in only one of six animals by 12 h after injection of PGF2 alpha. After PGF2 alpha injection, steady-state levels of follicular LHr were 4-fold lower (P < 0.05) and follicular FSHr mRNA levels were 2.4-fold lower (P < 0.05) in eCG- compared with FSH-treated cattle. In eCG-treated animals, induction of luteolysis led to a significant increase in follicular LHr mRNA levels (P < 0.01) and a significant decrease in follicular FSHr mRNA levels (P < 0.01). There was no such effect of luteolysis in FSH-treated animals. We conclude that superovulation with eCG, compared with FSH, results in lower follicular levels of LHr and FSHr mRNA but does not affect luteal LHr mRNA levels.
与使用促卵泡素（FSH）相比，用马绒毛膜促性腺激素（eCG）进行超排时，黄体促黄体生成素受体（LHr）以及卵泡促黄体生成素受体和促卵泡素受体（FSHr）的稳态mRNA水平更高。用eCG（n = 10）或FSH（n = 10）刺激小母牛，在用前列腺素F2α（PGF2α）诱导黄体溶解前一天以及诱导后12小时和24小时采集卵巢。从单个卵泡和黄体中纯化总RNA。采用同源cDNA探针通过狭缝印迹分析评估LHr和FSHr mRNA的稳态水平。在黄体溶解前，FSH刺激组和eCG刺激组动物的黄体LHr没有差异，并且在注射PGF2α后12小时，仅在六只动物中的一只检测到杂交信号。注射PGF2α后，与FSH处理的牛相比，eCG处理的牛卵泡LHr的稳态水平低4倍（P < 0.05），卵泡FSHr mRNA水平低2.4倍（P < 0.05）。在eCG处理的动物中，黄体溶解诱导导致卵泡LHr mRNA水平显著增加（P < 0.01），卵泡FSHr mRNA水平显著降低（P < 0.01）。在FSH处理的动物中没有黄体溶解的这种效应。我们得出结论，与FSH相比，用eCG进行超排会导致卵泡LHr和FSHr mRNA水平降低，但不影响黄体LHr mRNA水平。
