Yao T, Takashima K
Department of Applied Chemistry, College of Engineering, Osaka Prefecture University, Japan.
Biosens Bioelectron. 1998 Jan 1;13(1):67-73. doi: 10.1016/s0956-5663(97)00076-6.
A sol-gel silicate-based biosensor for glucose was made by utilizing a composite membrane of sol-gel enzyme film and electrochemically generated poly(1,2-diaminobenzene) film to improve the selectivity of the sol-gel enzyme sensors. The stability of the sensor was improved by exposing the enzyme layer to glutaraldehyde vapor. The glucose sensor responded rapidly (ca. 15 s) to glucose at 0.6 V (versus Ag/AgCl), without any interferences from electroactive species such as L-ascorbate and urate below 0.2 mM. Reliable results were obtained in the assays of glucose in controlled human sera, with both the steady-state and flow-injection measurements. Subsequently, the same sol-gel procedure was applied to the preparation of the sensors for galactose and cholesterol. The galactose and cholesterol sensors responded rapidly to galactose and cholesterol, respectively, although the sensitivity of these sensors was inferior to that of glucose sensor.
通过使用溶胶-凝胶酶膜和电化学合成的聚（1,2-二氨基苯）膜的复合膜来提高溶胶-凝胶酶传感器的选择性，制备了一种用于葡萄糖检测的基于溶胶-凝胶硅酸盐的生物传感器。通过将酶层暴露于戊二醛蒸汽中提高了传感器的稳定性。该葡萄糖传感器在0.6 V（相对于Ag/AgCl）下对葡萄糖响应迅速（约15秒），在0.2 mM以下不受诸如L-抗坏血酸盐和尿酸盐等电活性物质的干扰。在人体血清葡萄糖检测的稳态和流动注射测量中均获得了可靠的结果。随后，相同的溶胶-凝胶程序被应用于制备半乳糖和胆固醇传感器。半乳糖和胆固醇传感器分别对半乳糖和胆固醇响应迅速，尽管这些传感器的灵敏度低于葡萄糖传感器。
