van Zeijl C M, van de Kamp E H, Punt P J, Selten G C, Hauer B, van Gorcom R F, van den Hondel C A
TNO Nutrition and Food Research Institute, Department of Molecular Genetics and Gene Technology, Zeist, The Netherlands.
J Biotechnol. 1997 Jan 3;59(3):221-4. doi: 10.1016/s0168-1656(97)00170-3.
A method was developed to perform PCR directly on mycelial pellets or colonies treated with NOVOzym 234. The method allows rapid screening of large numbers of transformants of both sporulating and non-sporulating fungi for the presence of (co)transforming plasmid copies or for specific genetic modifications such as gene disruption and site specific integration. PCR fragments of at least 3.2 kb can be obtained. Using this method the identification of specific disruption mutants from Aspergillus niger and Beauveria bassiana was carried out.
开发了一种直接对用诺维信234处理的菌丝球或菌落进行PCR的方法。该方法可快速筛选大量产孢和不产孢真菌的转化体，以检测（共）转化质粒拷贝的存在或特定的基因修饰，如基因破坏和位点特异性整合。可获得至少3.2 kb的PCR片段。利用该方法对黑曲霉和球孢白僵菌的特定破坏突变体进行了鉴定。
