Cai W, Jing J, Irvin B, Ohler L, Rose E, Shizuya H, Kim U J, Simon M, Anantharaman T, Mishra B, Schwartz D C
W. M. Keck Laboratory for Biomolecular Imaging, Department of Chemistry, New York University, 31 Washington Place, New York, NY 10003, USA.
Proc Natl Acad Sci U S A. 1998 Mar 31;95(7):3390-5. doi: 10.1073/pnas.95.7.3390.
Large insert clone libraries have been the primary resource used for the physical mapping of the human genome. Research directions in the genome community now are shifting direction from purely mapping to large-scale sequencing, which in turn, require new standards to be met by physical maps and large insert libraries. Bacterial artificial chromosome libraries offer enormous potential as the chosen substrate for both mapping and sequencing studies. Physical mapping, however, has come under some scrutiny as being "redundant" in the age of large-scale automated sequencing. We report the development and applications of nonelectrophoretic, optical approaches for high-resolution mapping of bacterial artificial chromosome that offer the potential to complement and thereby advance large-scale sequencing projects.
大型插入片段克隆文库一直是人类基因组物理图谱构建的主要资源。目前，基因组学界的研究方向正从单纯的图谱构建转向大规模测序，这反过来又要求物理图谱和大型插入片段文库满足新的标准。细菌人工染色体文库作为图谱构建和测序研究的首选底物具有巨大潜力。然而，在大规模自动化测序时代，物理图谱构建受到了一些质疑，被认为是“多余的”。我们报告了用于细菌人工染色体高分辨率图谱构建的非电泳光学方法的开发和应用，这些方法有可能补充并推动大规模测序项目。
