Smith M W, Holmsen A L, Wei Y H, Peterson M, Evans G A
Molecular Genetics Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037.
Nat Genet. 1994 May;7(1):40-7. doi: 10.1038/ng0594-40.
We present a simple and efficient method for constructing high resolution physical maps of large regions of genomic DNA based upon sampled sequencing. The physical map is constructed by ordering high density cosmid contigs and determining a sequence fragment from each end of every clone. The resulting map, which contains 30-50% of the complete DNA sequence, allows the identification of many genes and makes possible PCR amplification of virtually any part of the genome. We apply this strategy to the automated analysis of the genome of the primitive eukaryote Giardia lamblia and evaluate its applicability to the physical mapping and DNA sequencing of the human genome.
我们提出了一种基于抽样测序构建基因组DNA大片段高分辨率物理图谱的简单有效方法。通过对高密度黏粒重叠群进行排序，并确定每个克隆两端的序列片段来构建物理图谱。所得图谱包含完整DNA序列的30%-50%，可用于鉴定许多基因，并使得对基因组几乎任何部分进行PCR扩增成为可能。我们将此策略应用于原始真核生物蓝氏贾第鞭毛虫基因组的自动化分析，并评估其在人类基因组物理图谱绘制和DNA测序中的适用性。
