使用重组主要表面蛋白5的酶联免疫吸附测定法对委内瑞拉牛无浆体病进行血清学诊断的评估。
Evaluation of an enzyme-linked immunosorbent assay using recombinant major surface protein 5 for serological diagnosis of bovine anaplasmosis in Venezuela.
作者信息
Reyna-Bello A, Cloeckaert A, Vizcaíno N, Gonzatti M I, Aso P M, Dubray G, Zygmunt M S
机构信息
Laboratoire de Pathologie Infectieuse et Immunologie, Institut National de la Recherche Agronomique, Nouzilly, France.
出版信息
Clin Diagn Lab Immunol. 1998 Mar;5(2):259-62. doi: 10.1128/CDLI.5.2.259-262.1998.
Abstract
An indirect enzyme-linked immunosorbent assay (ELISA) was developed for the serological diagnosis of bovine anaplasmosis with purified recombinant major surface protein 5 (MSP5) of Anaplasma marginale produced in Escherichia coli. Serum antibody responses against MSP5 were detected in calves experimentally infected with A. marginale as early as 21 days postinfection and reached maximum titers at 28 days postinfection. The MSP5 ELISA performed with serum samples taken from field cattle from different regions of Venezuela showed a seroprevalence of 47%, which seems to be in accordance with the reported epidemiological status of bovine anaplasmosis in Venezuela. Positive results obtained in the MSP5 ELISA were further confirmed by immunoblotting, with the recombinant MSP5 as the antigen. Thus, these results confirmed the importance of MSP5 as a suitable antigen for the serological diagnosis of bovine anaplasmosis.
摘要
利用在大肠杆菌中产生的纯化重组边缘无浆体主要表面蛋白5（MSP5），开发了一种间接酶联免疫吸附测定（ELISA）用于牛无浆体病的血清学诊断。在实验感染边缘无浆体的犊牛中，早在感染后21天就检测到了针对MSP5的血清抗体反应，并在感染后28天达到最高滴度。对从委内瑞拉不同地区的田间牛采集的血清样本进行的MSP5 ELISA检测显示，血清阳性率为47%，这似乎与委内瑞拉报道的牛无浆体病流行病学状况相符。以重组MSP5为抗原，通过免疫印迹进一步证实了MSP5 ELISA获得的阳性结果。因此，这些结果证实了MSP5作为牛无浆体病血清学诊断合适抗原的重要性。
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