Knowles D, Torioni de Echaide S, Palmer G, McGuire T, Stiller D, McElwain T
Animal Disease Research Unit, USDA Agricultural Research Service, Pullman, Washington, USA.
J Clin Microbiol. 1996 Sep;34(9):2225-30. doi: 10.1128/jcm.34.9.2225-2230.1996.
A protein epitope of major surface protein 5 (MSP5), defined by monoclonal antibody (MAb) ANAF16C1, is conserved among Anaplasma species (E. S. Visser, T. C. McGuire, G. H. Palmer, W. C. Davis, V. Shkap, E. Pipano, and D. P. Knowles, Jr., Infect. Immun. 60:5139-5144, 1992) and is expressed in the salivary glands of infected ticks. A competitive inhibition ELISA (cELISA) for the detection of bovine anti-MSP5 antibodies was developed by using purified recombinant MSP5 fusion protein and MAb ANAF16C1. The specificity of the recombinant-MSP5 cELISA within North America was established by using 261 serum samples from cattle in the regions of Hawaii and Northern Ontario where anaplasmosis is not endemic and from cattle proven by splenectomy or subinoculation of whole blood into susceptible splenectomized recipients to be uninfected. The maximum percent inhibition by these sera was 18%. Sera known to be positive were obtained from 35 cattle either experimentally inoculated with infected erythrocytes or exposed to infected Dermacentor andersoni ticks. Thirty-four of the 35 serum samples inhibited MAb ANAF16C1 binding by > or = 25%. During acute infection, the MSP5 cELISA detected antibodies prior to or concomitantly with the appearance of rickettsiae in erythrocytes. Antibodies were detectable in sera from persistently infected cattle inoculated as long as 6 years previously.
由单克隆抗体（MAb）ANAF16C1所界定的主要表面蛋白5（MSP5）的一种蛋白质表位，在无形体菌种中是保守的（E.S.维瑟、T.C.麦圭尔、G.H.帕尔默、W.C.戴维斯、V.什卡普、E.皮帕诺和小D.P.诺尔斯，《感染与免疫》60:5139 - 5144，1992年），并且在受感染蜱的唾液腺中表达。通过使用纯化的重组MSP5融合蛋白和MAb ANAF16C1，开发了一种用于检测牛抗MSP5抗体的竞争抑制酶联免疫吸附测定（cELISA）。利用来自夏威夷和安大略省北部地区牛的261份血清样本确定了北美地区重组MSP5 cELISA的特异性，在这些地区无形体病并非地方病，这些血清样本来自通过脾切除术或向易感脾切除受体接种全血证明未感染的牛。这些血清的最大抑制百分比为18%。已知阳性的血清取自35头牛，这些牛要么经实验接种了感染的红细胞，要么接触了感染的安德逊革蜱。35份血清样本中的34份抑制MAb ANAF16C1结合的程度≥25%。在急性感染期间，MSP5 cELISA在红细胞中出现立克次氏体之前或同时检测到抗体。在长达6年前接种的持续感染牛的血清中可检测到抗体。
